Trichoderma reesei

Technical Data

Type of microorganism	Fungus
Microorganism name	Trichoderma reesei
Average yield in organism	1-2 g/L (average v+B3+C1:L6+B3+C1:L6+B3+C1:L6+C1:O6+B3+C1:L+C1:U6
General temperature range	28°C (average value from studies cited before)
General pH range	pH 4.8 - 5.5 (average values from studies cited before)
Growth rate (µ)	0.12/hour (Hardy et al., 2017)
Ease of genetic modification	Genetic manipulation is more difficult than in yeast and bacteria; but genetic tools are widely characterized for T. reesei (Garvey, 2022)
Feedstock case studies (suitable substrates)	Orange peel (Thiviya et al., 2022) Birchwood chips and soybean hull (Alcantara & Mondala, 2021) Cane molasses (J. He et al., 2014)
Downstream purification processing complexity (isloation, lysis, purification) *	Produce a wide array of proteins and enzymes, what can make downstream processing more complex (Garvey, 2022) Foaming during femrnetation can lead to more complex DSP (Nakari-Setala et al., 2001) Possibilty of extracellular production, reducing DSP complexity (Chai et al., 2022)
Advantages	Very good secretion of proteins, mammalian-like PTM pathways: glycosylation, peptide chain shearing, and disulfide bond formation, similar to those of mammal cells. High protein yield (Garvey, 2022)
Challenges (Key limitations, risk factors)	Complex morpghological structures, which can be challenging for industrial scale-up. Co-secretion of native enzymes, which complex DSP and reduce yield (Garvey, 2022) Foaming during femrnetation can lead to more complex DSP (Nakari-Setala et al., 2001)
Extra/remark

Chymosin B (Uusitalo et al., 1991)

Lysozyme (Van Kimmenade et al., 2009)

Beta-lactoglobulin (Aro et al., 2023)

Ovalbumin (Aro et al., 2023)